Journal: Oncotarget

Article Title: MiR-384 inhibits human colorectal cancer metastasis by targeting KRAS and CDC42

doi: 10.18632/oncotarget.12704

Figure Lengend Snippet: A. Expression of miR-384 in six CRC cell lines and one normal colorectal mucosa cell line FHC were detected by real-time PCR. B. Over-expression of miR-384 in SW480 and HCT116 CRC cells verified by real-time PCR. C. Wound-healing assay. Histograms represent the average migrated distances at the indicated times. Error bars represent mean±s.d. from three independent experiments. D-E. The migratory and invasive properties of SW480/Vector, SW480/miR-384 and HCT116/Vector, HCT116/miR-384 cells were analyzed using Boyden chambers or Matrigel-coated Boyden chambers. Error bars represent mean±s.d. from three independent experiments. F. Three-dimensional morphologies assay. Histograms represent the average number of filopodia formed by each cell sphere from three independent experiments. Error bars represent mean±s.d.. G. Representative images of gross specimens of liver metastatic lesions formed in mice injected intrasplenically with SW480/Vector and SW480/miR-384. H. The number of liver visible metastatic nodules was shown ( ** p <0.01). (I) Overall survival of mice bearing liver metastases of SW480/Vector and SW480/miR-384 (log-rank test, n=8, ** p <0.01).

Article Snippet: The human CRC cell lines SW480, SW620, SW837, HCT116, HCT15, LOVO and normal colorectal mucosa cell FHC were obtained from American Type Culture Collection (ATCC).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Over Expression, Wound Healing Assay, Plasmid Preparation, Injection

Journal: Nature cell biology

Article Title: ATF4 couples MYC-dependent translational activity to bioenergetic demands during tumor progression

doi: 10.1038/s41556-019-0347-9

Figure Lengend Snippet: a. Immunoblot of B cells isolated from WT (n=3), pre-lymphoma Eμ-myc (n=3) or lymphoma bearing Eμ-myc mice (n=6), lysates from different mice per group were used. b . Immunoblot from two normal human B cells (NHBC) and Burkitt’s lymphoma cell lines. c. Immunoblot from normal human colonocytes (NHC) or colon cancer cell lines. d. Immunoblot from MCF10 (non-tumorigenic breast epithelial cell line) or breast cancer cell lines. e. Pearson correlation between EIF4EBP1 and ATF4 target gene expression in Diffused Large B cell Lymphoma (DLBCL, n=562), Colorectal Adenocarcinoma (COAD, n=329), Breast Cancer (BRCA, n=1218) and Sarcoma (SARC, n=265). The center lines depict linear regression lines and shaded regions are 95% confidence intervals for regression lines. Datasets analyzed are listed in the section. Previously known ATF4 target gene list used in this analysis is shown in table. f. Kaplan-Meier plots of progression free survival (left) and overall survival (right) of DLBCL patients with high or low EIF4EBP1 expression. The survival analysis using Kaplan-Meier and two-sided log-rank test between high and low EIF4EBP1 expression groups was performed on all the patients with records of progression-free survival (left, n= 173) and overall survival (right, n=171), respectively. g. Proposed model of ATF4 and MYC co-operation in tumor progression. All immunoblots are from n=1 and unprocessed scans of blots are shown in .

Article Snippet: Normal human colonocytes were purchased from Cell Applications Inc (732Cn-10a).

Techniques: Western Blot, Isolation, Targeted Gene Expression, Expressing